Anti-Dnmt1 Anticorpo [ARC51348] (A307587)

Name: Anti-Dnmt1 Antibody [ARC51348]
Brand: Antibodies.com
SKU: A307587
Price: 470.0 USD
Availability: InStock

Clicca sull'immagine per la visualizzazione estesa

$470

Anticorpo monoclonale [ARC51348] di coniglio contro Dnmt1 per WB, ICC/IF, IP e ChIP.

Garanzia al 100%

Garanzia di corrispondenza del prezzo

Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanzie

Garanzia di qualità al 100%

Garanzia di corrispondenza del prezzo

Descrizione Prodotto

Nome

Anti-Dnmt1 Antibody [ARC51348]

Descrizione

Rabbit monoclonal [ARC51348] antibody to Dnmt1.

Applicazioni

WB, ICC/IF, IP, ChIP

Diluizioni consigliate

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000, ChIP: 1:50-1:200

Reattività

Human, Mouse, Rat

Immunogeno

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DNMT1 (NP_001370.1).

Sequenza

MPARTAPARVPTLAVPAISLPDDVRRRLKDLERDSLTEKECVKEKLNLLHEFLQTEIKNQLCDLETKLRKEELSEEGYLAKVKSLLNKDLSLENGAHAYN

Specie ospite

Rabbit

Clonalità

Monoclonal

Clona ID

ARC51348

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Affinity purification.

Peso molecolare

200 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

ADCADN, AIM, CXXC finger protein 9, CXXC-type zinc finger protein 9, CXXC9, DNA (cytosine 5 ) methyltransferase 1, DNA (cytosine-5)-methyltransferase 1, DNA methyltransferase 1, DNA methyltransferase HsaI, DNA methyltransferase M.HsaI., DNA MTase, DNA MTase HsaI, DNMT, DNMT 1, Dnmt1o, DNMT1_HUMAN, FLJ16293, HSN1E, M.HsaI, MCMT, Met1, MGC104992, mMmul, MommeD2

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (4)

Clicca sull'immagine per la visualizzazione estesa

Figure 1: Western Blot - Anti-Dnmt1 Antibody [ARC51348] (A307587)

Western blot analysis of extracts of various cell lines, using Anti-Dnmt1 Antibody [ARC51348] (A307587) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

Clicca sull'immagine per la visualizzazione estesa

Figure 2: Western Blot - Anti-Dnmt1 Antibody [ARC51348] (A307587)

Western blot analysis of extracts from wild type (WT) and DNMT1 knockdown (KD) 293T cells, using Anti-Dnmt1 Antibody [ARC51348] (A307587) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

Clicca sull'immagine per la visualizzazione estesa

Figure 3: Immunofluorescence - Anti-Dnmt1 Antibody [ARC51348] (A307587)

Immunofluorescence analysis of C6 cells using Anti-Dnmt1 Antibody [ARC51348] (A307587). DAPI was used to stain the cell nuclei (blue).

Clicca sull'immagine per la visualizzazione estesa

Figure 4: Western Blot - Anti-Dnmt1 Antibody [ARC51348] (A307587)

Immunoprecipitation analysis of 300µg extracts of Jurkat cells using 3µg of Anti-Dnmt1 Antibody [ARC51348] (A307587). This Western blot was performed on the immunoprecipitate using Anti-Dnmt1 Antibody [ARC51348] (A307587) at a dilution of 1:1000.