Anti-Dnmt1 Anticorpo (A307715)

Name: Anti-Dnmt1 Antibody
Brand: Antibodies.com
SKU: A307715
Price: 335.0 USD
Availability: InStock

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$335

Anticorpo policlonale di coniglio contro Dnmt1 per WB, ICC/IF, IP e ChIP.

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanzie

Garanzia di qualità al 100%

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Descrizione Prodotto

Nome

Anti-Dnmt1 Antibody

Descrizione

Rabbit polyclonal antibody to Dnmt1.

Applicazioni

WB, ICC/IF, IP, ChIP

Diluizioni consigliate

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000, ChIP: 1:500-1:1,000

Reattività

Human

Immunogeno

Recombinant fusion protein containing a sequence corresponding to amino acids 1417-1616 of human DNMT1 (NP_001370.1).

Sequenza

MSALVAARMRHIPLAPGSDWRDLPNIEVRLSDGTMARKLRYTHHDRKNGRSSSGALRGVCSCVEAGKACDPAARQFNTLIPWCLPHTGNRHNHWAGLYGRLEWDGFFSTTVTNPEPMGKQGRVLHPEQHRVVSVRECARSQGFPDTYRLFGNILDKHRQVGNAVPPPLAKAIGLEIKLCMLAKARESASAKIKEEEAAKD

Specie ospite

Rabbit

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Affinity purification.

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

ADCADN, AIM, CXXC finger protein 9, CXXC-type zinc finger protein 9, CXXC9, DNA (cytosine 5 ) methyltransferase 1, DNA (cytosine-5)-methyltransferase 1, DNA methyltransferase 1, DNA methyltransferase HsaI, DNA methyltransferase M.HsaI., DNA MTase, DNA MTase HsaI, DNMT, DNMT 1, Dnmt1o, DNMT1_HUMAN, FLJ16293, HSN1E, M.HsaI, MCMT, Met1, MGC104992, mMmul, MommeD2

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (4)

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Figure 1: Western Blot - Anti-Dnmt1 Antibody (A307715)

Western blot analysis of extracts of various cell lines, using Anti-Dnmt1 Antibody (A307715) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Immunofluorescence - Anti-Dnmt1 Antibody (A307715)

Immunofluorescence analysis of 293T cells using Anti-Dnmt1 Antibody (A307715) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 3: Western Blot - Anti-Dnmt1 Antibody (A307715)

Immunoprecipitation analysis of 300µg extracts of Jurkat cells using 3µg of Anti-Dnmt1 Antibody (A307715). This Western blot was performed on the immunoprecipitate using Anti-Dnmt1 Antibody (A307715) at a dilution of 1:1000.

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Figure 4: Chromatin Immunoprecipitation - Anti-Dnmt1 Antibody (A307715)

Chromatin immunoprecipitation (ChIP) analysis of extracts of Jurkat cells, using Anti-Dnmt1 Antibody (A307715) and Rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.