DNA Polymerase alpha Cell Based Kit Elisa (A103593)

Nome
DNA Polymerase alpha Cell Based ELISA Kit
Tipo di kit
Cell-based (qualitative)
Principio di kit
This DNA Polymerase alpha Cell Based ELISA Kit allows for the detection of DNA Polymerase alpha and the effects that certain stimulation conditions have on DNA Polymerase alpha expression in different cell lines. Qualitative determination of DNA Polymerase alpha concentration is achieved by an indirect ELISA format. In essence, the DNA Polymerase alpha is captured by Anti-DNA Polymerase alpha Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this DNA Polymerase alpha Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Piattaforma
Microplate
Tipo di rilevamento
Colorimetric
Tipo di campione
Adherent cells and suspension cells.
Campo di rilevamento
> 5000 Cells
Tempo del kit
4 hours 30 minutes
Reattività
Human
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Conservazione
Store at + 4°C. Stable for 6 months.
Sinonimi
DKFZp686K1672, DNA polymerase alpha 1 catalytic subunit, DNA polymerase alpha catalytic subunit, DNA polymerase alpha catalytic subunit p180, DNA polymerase alpha p180 subunit, DPOLA_HUMAN, OTTHUMP00000023089, OTTHUMP00000023090, P180, p180 subunit, POLA, POLA1, Polymerase (DNA directed), alpha 1, catalytic subunit, Polymerase (DNA-directed), alpha (70kD)
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Inizio pagina