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Anti-DNA Photolyase Antibody (A101676)

Western blot of E. coli photolyase. 1. Purified Photolyase (8.4ng). 2. Purified Photolyase (42ng). 3. E. coli crude lysate (40µg). SDS-PAGE 12.5% gel. Blotting: 15 v, overnight, blocking by 5% skim milk. Anti-DNA Photolyase Antibody was used at 1:1,000 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H&L) was used at 1:10,000. Note that there are only 10-20 molecules of photolyase per E. coli cell. Immunoprecipitation of E. coli Photolyase. Immunoprecipitation was performed by using Dynabeads Magnetic system (Thermo Fisher). 1: E. coli AB1157 cell lysate (300µg), Anti-DNA Photolyase Antibody (2µg). 2: E. coli AB1157 cell lysate (300µg), Anti-DNA Photolyase Antibody (10µg). 3: Positve control: E. coli Photolyase purified (4.2µg), Anti-DNA Photolyase Antibody (10µg). 4: Negative control: No sample, Anti-DNA Photolyase Antibody (10µg). Western blot: Anti-DNA Photolyase Antibody was used at 1:1,000 dilution and the secondary antibody was Goat Anti-Rabbit IgG (HRP) used at 1:1,000 dilution.
$555

Rabbit polyclonal antibody to DNA Photolyase for WB, IP and ELISA.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 4-6 business days.

  • Specifications

Name
Anti-DNA Photolyase Antibody
Description
Rabbit polyclonal antibody to DNA Photolyase.
Applications
WB, IP, ELISA
Dilutions
WB: 1:1,000, IP: 1:200
Reactivity
Escherichia coli
Immunogen
Recombinant full length E. coli Cyclobutane Pyrimidine Dimer Photolyase.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Protein A chromatography.
Concentration
2 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol (filter sterilised and without Sodium Azide).
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Western blot of E. coli photolyase. 1. Purified Photolyase (8.4ng). 2. Purified Photolyase (42ng). 3. E. coli crude lysate (40µg). SDS-PAGE 12.5% gel. Blotting: 15 v, overnight, blocking by 5% skim milk. Anti-DNA Photolyase Antibody was used at 1:1,000 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H&L) was used at 1:10,000. Note that there are only 10-20 molecules of photolyase per E. coli cell.Enlarge Image

SDS-PAGE - Anti-DNA Photolyase Antibody (A101676)

Anti-DNA Photolyase Antibody
Immunoprecipitation of E. coli Photolyase. Immunoprecipitation was performed by using Dynabeads Magnetic system (Thermo Fisher). 1: E. coli AB1157 cell lysate (300µg), Anti-DNA Photolyase Antibody (2µg). 2: E. coli AB1157 cell lysate (300µg), Anti-DNA Photolyase Antibody (10µg). 3: Positve control: E. coli Photolyase purified (4.2µg), Anti-DNA Photolyase Antibody (10µg). 4: Negative control: No sample, Anti-DNA Photolyase Antibody (10µg). Western blot: Anti-DNA Photolyase Antibody was used at 1:1,000 dilution and the secondary antibody was Goat Anti-Rabbit IgG (HRP) used at 1:1,000 dilution.Enlarge Image

Immunoprecipitation - Anti-DNA Photolyase Antibody (A101676)

Anti-DNA Photolyase Antibody
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