Anti-DKK1 Anticorpo (A84041)

Name: Anti-DKK1 Antibody
Brand: Antibodies.com
SKU: A84041
Price: 480.0 USD
Availability: InStock

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$480

Anticorpo policlonale di capra contro DKK1 per ELISA, WB, IF, IHC e Flow Cytometry.

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Consegna

Spese di spedizione

$40

Tempi di consegna

3-5 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Descrizione Prodotto

Nome

Anti-DKK1 Antibody

Descrizione

Goat polyclonal antibody to DKK1.

Applicazioni

ELISA, WB, IF, IHC, Flow Cytometry

Diluizioni consigliate

ELISA: 1:128,000, WB: 1-3µg/ml, IHC: 10µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reattività

Human

Immunogeno

Synthetic peptide corresponding to the C terminal region of human DKK1.

Sequenza

CDHHQASNSSRLHT

Specie ospite

Goat

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentrazione

500 µg/ml

Peso molecolare

38 kDa

MW previsto

28.7kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Sinonimi

Dickkopf 1, Dickkopf 1 homolog, Dickkopf 1 like, Dickkopf homolog 1 , Dickkopf like protein 1, Dickkopf related protein 1, Dickkopf WNT signaling pathway inhibitor 1, Dickkopf-1, Dickkopf-related protein 1, DKK 1, Dkk-1, DKK1_HUMAN, hDkk 1 , hDkk-1, hDkk1 , mDkk1, SK

Controlli isotipo

Goat IgG (A121671)

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (4)

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Figure 1: Western Blot - Anti-DKK1 Antibody (A84041)

DKK1 expression in MCF7 cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-DKK1 Antibody (A84041) at 1µg/ml and detected by chemiluminescence.

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Figure 2: Immunohistochemistry - Anti-DKK1 Antibody (A84041)

DKK1 expression in Human Placenta analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-DKK1 Antibody (A84041) at 10µg/ml and revealed with alkaline phosphatase (AP).

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Figure 3: Immunofluorescence - Anti-DKK1 Antibody (A84041)

DKK1 expression in HepG2 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-DKK1 Antibody (A84041) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 4: Flow Cytometry - Anti-DKK1 Antibody (A84041)

DKK1 expression in HepG2 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-DKK1 Antibody (A84041) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.