Anti-DEP1 Antibody [MEM-CD148/05] (A281172)

Anti-DEP1 Antibody [MEM-CD148/05]

Mouse monoclonal [MEM-CD148/05] antibody to DEP1.

This antibody recognises CD148, a member of the receptor protein tyrosine phosphatase family. CD148 contains an ectodomain composed of multiple fibronectin type III-like repeats, a transmembrane domain and a single catalytic phosphatase domain. CD148 is broadly expressed including in platelets, T cells, B cells, macrophages as well as in some non-hematopoietic cells. CD148 functions as a key regulator of platelet reactivity via its regulation of Src family kinases (SFKs). Deletion of CD148 in mice has been shown to significantly impact platelet reactivity and thrombosis via SFK activity regulation. Clone MEM-CD148/05 has been used in immunofluorescence experiments to examine the co-localization of CD148 with CD45 at the immunological synapses formed between Tim-3hiCD8+ T cells.

Human

Human recombinant CD148 (amino acids 1-444).

MKPAAREARLPPRSPGLRWALPLLLLLLRLGQILCAGGTPSPIPDPSVATVATGENGITQISSTAESFHKQNGTGTPQVETNTSEDGESSGANDSLRTPEQGSNGTDGASQKTPSSTGPSPVFDIKAVSISPTNVILTWKSNDTAASEYKYVVKHKMENEKTITVVHQPWCNITGLRPATSYVFSITPGIGNETWGDPRVIKVITEPIPVSDLRVALTGVRKAALSWSNGNGTASCRVLLESIGSHEELTQDSRLQVNISGLKPGVQYNINPYLLQSNKTKGDPLGTEGGLDASNTERSRAGSPTAPVHDESLVGPVDPSSGQQSRDTEVLLVGLEPGTRYNATVYSQAANGTEGQPQAIEFRTNAIQVFDVTAVNISATSLTLIWKVSDNESSSNYTYKIHVAGETDSSNLNVSEPRAVIPGLRSSTFYNITVCPVLGDIEGT

Mouse

Monoclonal

MEM-CD148/05

IgG2b

Protein A affinity chromatography of tissue culture supernatant.

1 mg/ml

>95% (by SDS-PAGE).

Liquid

Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.

Store undiluted at 4°C. Do not freeze!

Mouse anti Human CD148 antibody, clone MEM-CD148/05 recognizes CD148, a member of the receptor protein tyrosine phosphatase family. CD148 contains an ectodomain composed of multiple fibronectin type III-like repeats, a transmembrane domain and a single catalytic phosphatase domain. CD148 is broadly expressed including in platelets, T cells, B cells, macrophages as well as in some non-hematopoietic cells. CD148 functions as a key regulator of platelet reactivity via its regulation of Src family kinases (SFKs) (Senis et al. 2009). Deletion of CD148 in mice has been shown to significantly impact platelet reactivity and thrombosis via SFK activity regulation (Ellison et al. 2010). Clone MEM-CD148/05 has been used in immunofluorescence experiments to examine the co-localization of CD148 with CD45 at the immunological synapses formed between Tim-3hiCD8+ T cells (Clayton et al. 2014).

CD148, Density-enhanced phosphatase 1, DEP-1, HPTP eta, Protein-tyrosine phosphatase eta, Protein-tyrosine phosphatase receptor type J, R-PTP-eta, R-PTP-J, Receptor-type tyrosine-protein phosphatase eta