Western Blot - Anti-Density Regulated Protein Antibody (A89155)
Western blot analysis of extracts of various cell lines, using Anti-Density Regulated Protein Antibody (A89155) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.
Immunohistochemistry - Anti-Density Regulated Protein Antibody (A89155)
Immunohistochemistry analysis of paraffin-embedded rat heart using Anti-Density Regulated Protein Antibody (A89155) at a dilution of 1:150 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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