Figure 1: Western Blot - Anti-DDAH1 Antibody (A82817)
DDAH1 expression in HepG2 (A) and LNCaP (B) cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-DDAH1 Antibody (A82817) at 0.3µg/ml and detected by chemiluminescence.
Figure 2: Western Blot - Anti-DDAH1 Antibody (A82817)
DDAH1 expression in Human Cerebellum (A), Kidney (B) and Testes (C) lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-DDAH1 Antibody (A82817) at 0.3µg/ml and detected by chemiluminescence.
DDAH1 expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-DDAH1 Antibody (A82817) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic, nuclear, and plasma membrane staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.
DDAH1 expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-DDAH1 Antibody (A82817) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.
DDAH1 expression in HeLa cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-DDAH1 Antibody (A82817) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.
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