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Anti-DARS2 Anticorpo (A10227)

Nome
Anti-DARS2 Antibody
Descrizione
Rabbit polyclonal antibody to DARS2.
Applicazioni
WB, IHC
Diluizioni consigliate
WB: 1:500-1:2,000, IHC: 1:50-1:200
Reattività
Human, Mouse
Immunogeno
Recombinant fusion protein containing a sequence corresponding to amino acids 406-645 of human DARS2 (NP_060592.2).
Sequenza
ANRNWNSPVANFIMESQRLELIRLMETQEEDVVLLTAGEHNKACSLLGKLRLECADLLETRGVVLRDPTLFSFLWVVDFPLFLPKEENPRELESAHHPFTAPHPSDIHLLYTEPKKARSQHYDLVLNGNEIGGGSIRIHNAELQRYILATLLKEDVKMLSHLLQALDYGAPPHGGIALGLDRLICLVTGSPSIRDVIAFPKSFRGHDLMSNTPDSVPPEELKPYHIRVSKPTDSKAERAH
Specie ospite
Rabbit
Clonalità
Polyclonal
Isotipo
IgG
Coniugare

Unconjugated

Purificazione
Affinity purification.
Peso molecolare
74 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
Aspartate tRNA ligase, Aspartate tRNA ligase 2 mitochondrial, Aspartate tRNA ligase mitochondrial, Aspartate--tRNA ligase, Aspartyl tRNA synthetase 2 (mitochondrial), Aspartyl tRNA synthetase mitochondrial, Aspartyl-tRNA synthetase, AspRS, FLJ10514, LBSL, mitochondrial, MT ASPRS, RP3 383J4.2, SYDM_HUMAN
Controlli isotipo
Secondari adatti
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Western Blot - Anti-DARS2 Antibody (A10227) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 1: Western Blot - Anti-DARS2 Antibody (A10227)
Western blot analysis of extracts of various cell lines, using Anti-DARS2 Antibody (A10227) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.
Western Blot - Anti-DARS2 Antibody (A10227) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 2: Western Blot - Anti-DARS2 Antibody (A10227)
Western blot analysis of extracts of various cell lines, using Anti-DARS2 Antibody (A10227) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.
Immunohistochemistry - Anti-DARS2 Antibody (A10227) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 3: Immunohistochemistry - Anti-DARS2 Antibody (A10227)
Immunohistochemistry analysis of paraffin-embedded human liver damage using Anti-DARS2 Antibody (A10227) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-DARS2 Antibody (A10227) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 4: Immunohistochemistry - Anti-DARS2 Antibody (A10227)
Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-DARS2 Antibody (A10227) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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