Figure 1: Western Blot - Anti-DARC Antibody (A82945)
DARC expression in Human Liver lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-DARC Antibody (A82945) at 0.3µg/ml and detected by chemiluminescence.
DARC expression in Human Lung analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by microwaving in citrate buffer, pH 6. Staining was performed with Anti-DARC Antibody (A82945) at 5µg/ml and revealed with alkaline phosphatase (AP).
DARC expression in human kidney analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by microwaving in citrate buffer, pH 6. Staining was performed with Anti-DARC Antibody (A82945) at 3µg/ml and revealed with horseradish peroxidase (HRP).
DARC expression in HEK293 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-DARC Antibody (A82945) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 0.4µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.
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