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Anti-Cytokeratin 8 Anticorpo (A12853)

Western Blot - Anti-Cytokeratin 8 Antibody (A12853) - Antibodies.com Western Blot - Anti-Cytokeratin 8 Antibody (A12853) - Antibodies.com Immunohistochemistry - Anti-Cytokeratin 8 Antibody (A12853) - Antibodies.com Immunohistochemistry - Anti-Cytokeratin 8 Antibody (A12853) - Antibodies.com Immunofluorescence - Anti-Cytokeratin 8 Antibody (A12853) - Antibodies.com
$335
Product Datasheet

Anticorpo policlonale di coniglio contro Cytokeratin 8 per WB, IHC e ICC/IF.

Garanzia al 100%
Garanzia di corrispondenza del prezzo

Spese di spedizione
$40
Tempi di consegna
5-8 Giorni lavorativi

Telefono
+1 (314) 370-6046
Lun - Ven, 8:00 - 16:00 AST
E-mail
orders@antibodies.com

Nome
Anti-Cytokeratin 8 Antibody
Descrizione
Rabbit polyclonal antibody to Cytokeratin 8.
Applicazioni
WB, IHC, ICC/IF
Diluizioni consigliate
WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reattività
Human, Mouse, Rat
Immunogeno
Recombinant fusion protein containing a sequence corresponding to amino acids 1-483 of human Cytokeratin 8 (KRT8) (NP_002264.1).
Sequenza
MSIRVTQKSYKVSTSGPRAFSSRSYTSGPGSRISSSSFSRVGSSNFRGGLGGGYGGASGMGGITAVTVNQSLLSPLVLEVDPNIQAVRTQEKEQIKTLNNKFASFIDKVRFLEQQNKMLETKWSLLQQQKTARSNMDNMFESYINNLRRQLETLGQEKLKLEAELGNMQGLVEDFKNKYEDEINKRTEMENEFVLIKKDVDEAYMNKVELESRLEGLTDEINFLRQLYEEEIRELQSQISDTSVVLSMDNSRSLDMDSIIAEVKAQYEDIANRSRAEAESMYQIKYEELQSLAGKHGDDLRRTKTEISEMNRNISRLQAEIEGLKGQRASLEAAIADAEQRGELAIKDANAKLSELEAALQRAKQDMARQLREYQELMNVKLALDIEIATYRKLLEGEESRLESGMQNMSIHTKTTSGYAGGLSSAYGGLTSPGLSYSLGSSFGSGAGSSSFSRTSSSRAVVVKKIETRDGKLVSESSDVLPK
Specie ospite
Rabbit
Clonalità
Polyclonal
Isotipo
IgG
Coniugare

Unconjugated

Purificazione
Affinity purification.
Peso molecolare
54 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
CARD2, CK 8, CK-8, CK8, CYK8, CYKER, Cytokeratin endo A, Cytokeratin-8, DreK8, EndoA, K2C8, K2C8_HUMAN, K8, Keratin 8, Keratin type II cytoskeletal 8, Keratin, type II cytoskeletal 8, Keratin-8, KO, Krt 2.8, KRT8, MGC118110, MGC174782, MGC53564, MGC85764, sb:cb186, Type-II keratin Kb8
Controlli isotipo
Secondari adatti
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Western Blot - Anti-Cytokeratin 8 Antibody (A12853) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 1: Western Blot - Anti-Cytokeratin 8 Antibody (A12853)
Western blot analysis of extracts of various cell lines, using Anti-Cytokeratin 8 Antibody (A12853) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Western Blot - Anti-Cytokeratin 8 Antibody (A12853) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 2: Western Blot - Anti-Cytokeratin 8 Antibody (A12853)
Western blot analysis of extracts of Rat liver, using Anti-Cytokeratin 8 Antibody (A12853) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry - Anti-Cytokeratin 8 Antibody (A12853) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 3: Immunohistochemistry - Anti-Cytokeratin 8 Antibody (A12853)
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Cytokeratin 8 Antibody (A12853) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-Cytokeratin 8 Antibody (A12853) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 4: Immunohistochemistry - Anti-Cytokeratin 8 Antibody (A12853)
Immunohistochemistry analysis of paraffin-embedded human liver using Anti-Cytokeratin 8 Antibody (A12853) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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