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Anti-Cytokeratin 20 Anticorpo [ARC0288] (A306519)

Western Blot - Anti-Cytokeratin 20 Antibody [ARC0288] (A306519) - Antibodies.com Western Blot - Anti-Cytokeratin 20 Antibody [ARC0288] (A306519) - Antibodies.com Immunohistochemistry - Anti-Cytokeratin 20 Antibody [ARC0288] (A306519) - Antibodies.com
$470
Product Datasheet

Anticorpo monoclonale [ARC0288] di coniglio contro Cytokeratin 20 per WB e IHC.

Garanzia al 100%
Garanzia di corrispondenza del prezzo

Spese di spedizione
$40
Tempi di consegna
5-8 Giorni lavorativi

Telefono
+1 (314) 370-6046
Lun - Ven, 8:00 - 16:00 AST
E-mail
orders@antibodies.com

Nome
Anti-Cytokeratin 20 Antibody [ARC0288]
Descrizione
Rabbit monoclonal [ARC0288] antibody to Cytokeratin 20.
Applicazioni
WB, IHC
Diluizioni consigliate
WB: 1:500-1:2,000, IHC: 1:50-1:200
Reattività
Human, Rat
Immunogeno
A synthetic peptide corresponding to a sequence within amino acids 325-424 of human Cytokeratin 20 (KRT20) (P35900).
Sequenza
LANLQSLLSSLEAQLMQIRSNMERQNNEYHILLDIKTRLEQEIATYRRLLEGEDVKTTEYQLSTLEERDIKKTRKIKTVVQEVVDGKVVSSEVKEVEENI
Specie ospite
Rabbit
Clonalità
Monoclonal
Clona ID
ARC0288
Isotipo
IgG
Coniugare

Unconjugated

Purificazione
Affinity purification.
Peso molecolare
44 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
CD20, CK 20, CK-20, CK20, Cytokeratin-20, Cytokeratin20, K1C20_HUMAN, K20, KA20, Keratin, Keratin 20, keratin 20, type I, keratin 21, rat, homolog of, Keratin type I cytoskeletal 20, Keratin-20, Keratin20, KRT 20, KRT 21, KRT20, KRT21, MGC35423, OTTHUMP00000164518, Protein IT, type I cytoskeletal 20
Controlli isotipo
Secondari adatti
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Western Blot - Anti-Cytokeratin 20 Antibody [ARC0288] (A306519) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 1: Western Blot - Anti-Cytokeratin 20 Antibody [ARC0288] (A306519)
Western blot analysis of extracts of HT-29 cells, using Anti-Cytokeratin 20 Antibody [ARC0288] (A306519) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Western Blot - Anti-Cytokeratin 20 Antibody [ARC0288] (A306519) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 2: Western Blot - Anti-Cytokeratin 20 Antibody [ARC0288] (A306519)
Western blot analysis of extracts of Rat liver, using Anti-Cytokeratin 20 Antibody [ARC0288] (A306519) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 3min.
Immunohistochemistry - Anti-Cytokeratin 20 Antibody [ARC0288] (A306519) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 3: Immunohistochemistry - Anti-Cytokeratin 20 Antibody [ARC0288] (A306519)
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Cytokeratin 20 Antibody [ARC0288] (A306519) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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