Figure 1: Western Blot - Anti-CASP Antibody (A83821)
CASP expression in Jurkat (A) and MOLT4 (B) cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary incubation was performed with Anti-CASP Antibody (A83821) at 0.5µg/ml (A) or 1µg/ml (B) and detected by chemiluminescence.
CASP expression in A431 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-CASP Antibody (A83821) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.
CASP expression in U251 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-CASP Antibody (A83821) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Strong cytoplasmic and weak nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.
