Western blot analysis of extracts of various cell lines, using Anti-CYP11A1 Antibody (A13608) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Western blot analysis of extracts of various cell lines, using Anti-CYP11A1 Antibody (A13608) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.
Western blot analysis of extracts of HeLa cells, using Anti-CYP11A1 Antibody (A13608) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-CYP11A1 Antibody (A13608) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of human placenta using Anti-CYP11A1 Antibody (A13608) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of rat adrenal gland using Anti-CYP11A1 Antibody (A13608) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of mouse adrenal gland using Anti-CYP11A1 Antibody (A13608) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
![Western Blot - Anti-CYP11A1 Antibody [ARC61692] (A329299) - Antibodies.com](https://cdn.antibodies.com/image/catalog/329/A329299_1.jpg?profile=product_alternative)
