Rabbit monoclonal [ARC1559] antibody to Cyclin E1 (phospho Thr77).
Applicazioni
WB
Diluizioni consigliate
WB: 1:500-1:2,000
Reattività
Human, Mouse
Immunogeno
A synthetic phosphorylated peptide around T77 of human Cyclin E1 (P24864).
Sequenza
IPTPD
Specie ospite
Rabbit
Clonalità
Monoclonal
Clona ID
ARC1559
Isotipo
IgG
Coniugare
Unconjugated
Purificazione
Affinity purification.
Peso molecolare
54 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
CCNE, Ccne1, CCNE1_HUMAN, cyclin E variant ex5del, cyclin E variant ex7del, Cyclin Es, Cyclin Et, CyclinE, G1/S specific cyclin E, G1/S-specific cyclin-E1
Figure 1: Western Blot - Anti-Cyclin E1 (phospho Thr77) Antibody [ARC1559] (A306326)
Western blot analysis of extracts of NIH/3T3 cells, using Anti-Cyclin E1 (phospho Thr77) Antibody [ARC1559] (A306326) at 1:1,000 dilution. NIH/3T3 cells were treated by Hydroxyurea (4 Mm) at 37°C for 20 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 3min.
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