Rabbit polyclonal antibody to Cyclin E1 (phospho Thr395).
Applicazioni
WB, ICC/IF
Diluizioni consigliate
WB: 1:500-1:2,000, ICC/IF: 1:50-1:200
Reattività
Human, Mouse, Rat
Immunogeno
A synthetic phosphorylated peptide around T395 of human Cyclin E1 (NP_001229.1).
Sequenza
LLTPP
Specie ospite
Rabbit
Clonalità
Polyclonal
Isotipo
IgG
Coniugare
Unconjugated
Purificazione
Affinity purification.
Peso molecolare
55 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
CCNE, Ccne1, CCNE1_HUMAN, cyclin E variant ex5del, cyclin E variant ex7del, Cyclin Es, Cyclin Et, CyclinE, G1/S specific cyclin E, G1/S-specific cyclin-E1
Figure 1: Western Blot - Anti-Cyclin E1 (phospho Thr395) Antibody (A10865)
Western blot analysis of extracts of various cell lines, using Anti-Cyclin E1 (phospho Thr395) Antibody (A10865). The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA.
Immunofluorescence analysis of C6 cells using Anti-Cyclin E1 (phospho Thr395) Antibody (A10865) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of NIH/3T3 cells using Anti-Cyclin E1 (phospho Thr395) Antibody (A10865) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of U2OS cells using Anti-Cyclin E1 (phospho Thr395) Antibody (A10865) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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