Rabbit polyclonal antibody to Cyclin B1 (phospho-Ser147)
Specificità
The antibody detects endogenous level of Cyclin B1 only when phosphorylated at serine 147.
Applicazioni
WB, IHC, IF
Reattività
Human
Immunogeno
Peptide sequence around phosphorylation site of Serine 147 (A-F-S(p)-D-V) derived from Human Cyclin B1.
Specie ospite
Rabbit
Clonalità
Polyclonal
Coniugare
Unconjugated
Purificazione
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentrazione
1 mg/ml
Formulazione
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Conservazione
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Cyclin B1 (Phospho-Ser147) Antibody #11540 (left) or the same antibody preincubated with blocking peptide (right).
![Immunohistochemistry - Anti-Cyclin B1 Antibody [CCNB1/1098] (A250460) - Antibodies.com](https://cdn.antibodies.com/image/catalog/250/A250460_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-Cyclin B1 Antibody [CCNB1/1098] - BSA and Azide free (A253640) - Antibodies.com](https://cdn.antibodies.com/image/catalog/253/A253640_1.jpg?profile=product_alternative)
![SDS-PAGE - Anti-Cyclin B1 Antibody [V92.1] (A250459) - Antibodies.com](https://cdn.antibodies.com/image/catalog/250/A250459_1.jpg?profile=product_alternative)
![SDS-PAGE - Anti-Cyclin B1 Antibody [V92.1] - BSA and Azide free (A253639) - Antibodies.com](https://cdn.antibodies.com/image/catalog/253/A253639_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-Cyclin B1 Antibody [SPM619] (A250461) - Antibodies.com](https://cdn.antibodies.com/image/catalog/250/A250461_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-Cyclin B1 Antibody [SPM619] - BSA and Azide free (A253641) - Antibodies.com](https://cdn.antibodies.com/image/catalog/253/A253641_1.jpg?profile=product_alternative)