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Anti-Cyclin B1 Anticorpo [ARC0474] (A306720)

Western Blot - Anti-Cyclin B1 Antibody [ARC0474] (A306720) - Antibodies.com Western Blot - Anti-Cyclin B1 Antibody [ARC0474] (A306720) - Antibodies.com
Knockout Convalidato
$470
Product Datasheet

Anticorpo monoclonale [ARC0474] di coniglio contro Cyclin B1 per WB.

Garanzia al 100%
Garanzia di corrispondenza del prezzo

Spese di spedizione
$40
Tempi di consegna
5-8 Giorni lavorativi

Telefono
+1 (314) 370-6046
Lun - Ven, 8:00 - 16:00 AST
E-mail
orders@antibodies.com

Nome
Anti-Cyclin B1 Antibody [ARC0474]
Descrizione
Rabbit monoclonal [ARC0474] antibody to Cyclin B1.
Applicazioni
WB
Diluizioni consigliate
WB: 1:500-1:2,000
Reattività
Human, Mouse
Immunogeno
Recombinant fusion protein containing a sequence corresponding to amino acids 3-177 of human Cyclin B1 (P14635).
Sequenza
LRVTRNSKINAENKAKINMAGAKRVPTAPAATSKPGLRPRTALGDIGNKVSEQLQAKMPMKKEAKPSATGKVIDKKLPKPLEKVPMLVPVPVSEPVPEPEPEPEPEPVKEEKLSPEPILVDTASPSPMETSGCAPAEEDLCQAFSDVILAVNDVDAEDGADPNLCSEYVKDIYAY
Specie ospite
Rabbit
Clonalità
Monoclonal
Clona ID
ARC0474
Isotipo
IgG
Coniugare

Unconjugated

Purificazione
Affinity purification.
Peso molecolare
55 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
CCNB, CCNB 1, ccnb1, CCNB1_HUMAN, G2 mitotic specific cyclin B1, G2/mitotic-specific cyclin-B1
Controlli isotipo
Secondari adatti
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Western Blot - Anti-Cyclin B1 Antibody [ARC0474] (A306720) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 1: Western Blot - Anti-Cyclin B1 Antibody [ARC0474] (A306720)
Western blot analysis of extracts from wild type (WT) and Cyclin B1 knockout (KO) HeLa cells, using Anti-Cyclin B1 Antibody [ARC0474] (A306720) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.
Western Blot - Anti-Cyclin B1 Antibody [ARC0474] (A306720) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 2: Western Blot - Anti-Cyclin B1 Antibody [ARC0474] (A306720)
Western blot analysis of extracts of various cell lines, using Anti-Cyclin B1 Antibody [ARC0474] (A306720) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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