Cyanine 3 maleimide (A270145)

Cyanine 3 maleimide

Thiol mono-reactive Cyanine 3 dye. This reagent can be used to attach Cyanine 3 fluorophore (an analog of Cy3®) to proteins and peptides containing cysteine residues, as well as to other thiolated molecules (such as thiol-containing oligonucleotides). Cystines should be reduced with TCEP (tris-carboxyethylphosphine) or other appropriate reductant prior to the labeling. Labeling with Cyanine 3 maleimide is selective, and efficient. We recommend using water-soluble Sulfo-Cyanine 3 maleimide for the labeling of antibodies and other sensitive proteins.

555 nm

150000 M^-1cm^-1

570 nm

0.31

0.09

95% (by ¹H NMR and HPLC-MS).

C₃₆H₄₃N₄O₃BF₄

666.56 Da

Red powder.

Well soluble in DMSO (0.50 M = 330 g/L), DMF, and dichloromethane. Very poorly soluble in water (0.57 mM = 420 mg/L).

Shipped at room temperature. Upon delivery, store in the dark at -20°C. Avoid prolonged exposure to light. Desiccate.

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.