Anti-CTCF Anticorpo (A307225)

Name: Anti-CTCF Antibody
Brand: Antibodies.com
SKU: A307225
Price: 470.0 USD
Availability: InStock

Clicca sull'immagine per la visualizzazione estesa

$470

Anticorpo policlonale di coniglio contro CTCF per WB, IHC e ICC/IF.

Garanzia al 100%

Garanzia di corrispondenza del prezzo

Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanzie

Garanzia di qualità al 100%

Garanzia di corrispondenza del prezzo

Tag dei prodotti:

Anticorpo Knockout Convalidato

Descrizione Prodotto

Nome

Anti-CTCF Antibody

Descrizione

Rabbit polyclonal antibody to CTCF.

Applicazioni

WB, IHC, ICC/IF

Diluizioni consigliate

WB: 1:100-1:500, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reattività

Human, Mouse, Rat

Immunogeno

Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human CTCF (NP_006556.1).

Sequenza

MEGDAVEAIVEESETFIKGKERKTYQRRREGGQEEDACHLPQNQTDGGEVVQDVNSSVQMVMMEQLDPTLLQMKTEVMEGTVAPEAEAAVDDTQIITLQVVNMEEQPINIGELQLVQVPVPVTVPVATTSVEELQGAYENEVSKEGLAESEPMICHTLPLPEGFQVVKVGANGEVETLEQGELPPQEDPSWQKDPDYQPPAKKTKKTKKSKLRYTEEGKDVDVSVYDFEEEQQEGLLSEVNAEKVVGNMKPPKPTKIKKK

Specie ospite

Rabbit

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Affinity purification.

Peso molecolare

140 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

11 zinc finger protein, 11 zinc finger transcriptional repressor, 11-zinc finger protein, CCCTC binding factor, CCCTC binding factor (zinc finger protein), CCCTC-binding factor, CTCFL paralog, CTCF_HUMAN, MRD21, Transcriptional repressor CTCF

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (5)

Clicca sull'immagine per la visualizzazione estesa

Figure 1: Western Blot - Anti-CTCF Antibody (A307225)

Western blot analysis of extracts of various cell lines, using Anti-CTCF Antibody (A307225) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

Clicca sull'immagine per la visualizzazione estesa

Figure 2: Western Blot - Anti-CTCF Antibody (A307225)

Western blot analysis of extracts of NIH/3T3 cells, using Anti-CTCF Antibody (A307225) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

Clicca sull'immagine per la visualizzazione estesa

Figure 3: Western Blot - Anti-CTCF Antibody (A307225)

Western blot analysis of extracts from wild type (WT) and CTCF knockout (KO) 293T cells, using Anti-CTCF Antibody (A307225) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

Clicca sull'immagine per la visualizzazione estesa

Figure 4: Immunohistochemistry - Anti-CTCF Antibody (A307225)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-CTCF Antibody (A307225) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.