This antibody recognises human corticotrophin releasing factor (CRF), a hormone found mainly in the hypothalamus that regulates the release of corticotrophin from the pituitary gland.The unique lysine residue (Lys-36) is likely to be involved in the antigenic determinant as lysine modification by CA blocks binding completely. Human serum, containing CRF-binding protein, also inhibits the binding of this antibody to CRF.
Applicazioni
ELISA, IHC-Fr, IHC-P, IF
Diluizioni consigliate
IHC-P 1: 1:100 - 1:7,000
Reattività
Human
Immunogeno
Corticotrophin Releasing Factor conjugated to Bovine Serum Albumin.
Specie ospite
Mouse
Clonalità
Monoclonal
Clona ID
4H9
Isotipo
IgG1
Coniugare
Unconjugated
Purificazione
Protein G affinity chromatography of ascites.
Concentrazione
1 mg/ml
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.
Conservazione
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
Note generali
Mouse anti Human Corticotrophin Releasing Factor antibody, clone 4H9 recognizes human corticotrophin releasing factor (CRF), a hormone found mainly in the hypothalamus that regulates the release of corticotrophin from the pituitary gland.The unique lysine residue (Lys-36) is likely to be involved in the antigenic determinant as lysine modification by CA blocks binding completely. Human serum, containing CRF-binding protein, also inhibits the binding of this antibody to CRF.