Anti-CREB (phospho Ser133) Anticorpo (A10929)

Name: Anti-CREB (phospho Ser133) Antibody
Brand: Antibodies.com
SKU: A10929
Price: 435.0 USD
Availability: InStock

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$435

Anticorpo policlonale di coniglio contro CREB (phospho Ser133) per WB e IHC.

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Descrizione Prodotto

Nome

Anti-CREB (phospho Ser133) Antibody

Descrizione

Rabbit polyclonal antibody to CREB (phospho Ser133).

Applicazioni

WB, IHC

Diluizioni consigliate

WB: 1:500-1:1,000, IHC: 1:50-1:100

Reattività

Human, Mouse, Rat

Immunogeno

A synthetic phosphorylated peptide around S133 of human CREB1 (NP_604391.1).

Sequenza

RPSYR

Specie ospite

Rabbit

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Affinity purification.

Peso molecolare

40 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

Active transcription factor CREB, cAMP response element binding protein, cAMP response element binding protein 1, cAMP responsive element binding protein 1, cAMP-responsive element-binding protein 1, CREB-1, CREB1, CREB1_HUMAN, Cyclic AMP-responsive element-binding protein 1, MGC9284, OTTHUMP00000163864, OTTHUMP00000163865, OTTHUMP00000206660, OTTHUMP00000206662, OTTHUMP00000206667, Transactivator protein

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (4)

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Figure 1: Western Blot - Anti-CREB (phospho Ser133) Antibody (A10929)

Western blot analysis of extracts of NIH/3T3 cells, using Anti-CREB (phospho Ser133) Antibody (A10929) at 1:1,000 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. NIH/3T3 cells were treated by Anisomycin (25 ug/ml) at 37°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Immunohistochemistry - Anti-CREB (phospho Ser133) Antibody (A10929)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-CREB (phospho Ser133) Antibody (A10929) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-CREB (phospho Ser133) Antibody (A10929)

Immunohistochemistry analysis of paraffin-embedded human tonsil using Anti-CREB (phospho Ser133) Antibody (A10929) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-CREB (phospho Ser133) Antibody (A10929)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-CREB (phospho Ser133) Antibody (A10929) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.