Rabbit polyclonal antibody to Cortactin (Phospho-Tyr466)
Specificità
The antibody detects endogenous level of Cortactin only when phosphorylated at tyrosine 466.
Applicazioni
WB
Diluizioni consigliate
Western blotting: 1:500~1:1000
Reattività
Human, Mouse
Immunogeno
Peptide sequence around phosphorylation site of tyrosine 466 (P-V-Y(p)-E-T) derived from Human CORTACTIN.
Specie ospite
Rabbit
Clonalità
Polyclonal
Coniugare
Unconjugated
Purificazione
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentrazione
1.0mg / ml
MW previsto
85kd
Formulazione
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Conservazione
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Western blot analysis of extracts from JK cells, treated with H2O2 or calf intestinal phosphatase (CIP), using Cortactin (Phospho-Tyr466) Antibody #11272.
Prodotti alternativi a Anti-Cortactin (Phospho-Tyr466) Anticorpo (A50758)