Anti-COL1A2 Antibody (A17214)

Name: Anti-COL1A2 Antibody
Brand: Antibodies.com
SKU: A17214
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to COL1A2 for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-COL1A2 Antibody

Description

Rabbit polyclonal antibody to COL1A2.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:25-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 500-600 of human Collagen I/COL1A2 (NP_000080.2).

Sequence

PTGDPGKNGDKGHAGLAGARGAPGPDGNNGAQGPPGPQGVQGGKGEQGPPGPPGFQGLPGPSGPAGEVGKPGERGLHGEFGLPGPAGPRGERGPPGESGAA

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

129 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Alpha-2 type I collagen, Collagen alpha-2(I) chain

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-COL1A2 Antibody (A17214)

Western blot analysis of extracts of A375 cells, using Anti-COL1A2 Antibody (A17214) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

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Immunohistochemistry - Anti-COL1A2 Antibody (A17214)

Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-COL1A2 Antibody (A17214) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-COL1A2 Antibody (A17214)

Immunofluorescence analysis of A-431 cells using Anti-COL1A2 Antibody (A17214) at a dilution of 1:25. DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-COL1A2 Antibody (A17214)

Immunofluorescence analysis of NIH/3T3 cells using Anti-COL1A2 Antibody (A17214) at a dilution of 1:25. DAPI was used to stain the cell nuclei (blue).

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