Rabbit polyclonal antibody to cofilin (Phospho-Ser3)
Specificità
The antibody detects endogenous level of cofilin only when phosphorylated at serine 3.
Applicazioni
WB, IHC, IF
Reattività
Human, Mouse, Rat
Immunogeno
Peptide sequence around phosphorylation site of serine 3 (M-A-S(p)-G-V) derived from Human cofilin.
Specie ospite
Rabbit
Clonalità
Polyclonal
Coniugare
Unconjugated
Purificazione
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentrazione
1.0mg / ml
Formulazione
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Conservazione
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Sinonimi
18 kDa phosphoprotein, CFL, CFL 1, CFL1, COF1_HUMAN, Cofilin 1, Cofilin 1 non muscle, Cofilin non muscle isoform, Cofilin-1, epididymis secretory protein Li 15, HEL-S-15, non-muscle isoform, p18
Western blot analysis of extracts from COLO205 cells using cofilin (Phospho-Ser3) Antibody #11139 (Lane 2) and the same antibody preincubated with blocking peptide (Lane1).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cofilin (Phospho-Ser3) Antibody #11139 (left) or the same antibody preincubated with blocking peptide (right).