Anti-Choline Acetyltransferase Anticorpo (A304944)

Name: Anti-Choline Acetyltransferase Antibody
Brand: Antibodies.com
SKU: A304944
Price: 500.0 USD
Availability: InStock

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$500

Anticorpo policlonale di coniglio contro Choline Acetyltransferase per WB e ICC/IF.

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Descrizione Prodotto

Nome

Anti-Choline Acetyltransferase Antibody

Descrizione

Rabbit polyclonal antibody to Choline Acetyltransferase.

Specificità

Predicted molecular weight at ~82.5 kDa. Observed molecular weights between 68-70 kDa.

Applicazioni

WB, ICC/IF

Diluizioni consigliate

WB: 1:1,000, ICC/IF: 1:100

Reattività

Human, Mouse

Immunogeno

Synthetic peptide from the N-terminal to the mid-protein of human Choline O-Acetyltrasferase.

Specie ospite

Rabbit

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Peptide affinity purification.

Concentrazione

1 mg/ml

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline with 50% Glycerol and 0.09% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

Acetyl CoA choline O acetyltransferase, Acetyl CoA:choline O acetyltransferase, ChAT, CHOACTase, Choline acetylase, Choline O acetyltransferase, Choline O-acetyltransferase, CLAT_HUMAN, CMS1A, CMS1A2, EC 2.3.1.6, OTTHUMP00000019583, OTTHUMP00000019584

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Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (2)

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Figure 1: Western Blot - Anti-Choline Acetyltransferase Antibody (A304944)

Western blot analysis of mouse brain showing detection of ~82.5kDa Choline Acetyltransferase protein using Anti-Choline Acetyltransferase Antibody (A304944) at 1:1,000 for 1 hour at room temperature. Lane 1: MW Ladder. Lane 2: mouse brain (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at room temperature. The secondary antibody used was Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at room temperature. Color Development: TMB solution for 12 min at room temperature. Predicted/Observed Size: ~82.5kDa.

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Figure 2: Immunofluorescence - Anti-Choline Acetyltransferase Antibody (A304944)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-Choline Acetyltransferase Antibody (A304944), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Rabbit ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60min room temperature, 5min room temperature. Localization: Nucleus. Magnification: 60X.(A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Choline Acetyltransferase Antibody (D) Composite.