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Anti-CHCHD2 Anticorpo (A88474)

Nome
Anti-CHCHD2 Antibody
Descrizione
Rabbit polyclonal antibody to CHCHD2.
Applicazioni
WB, IHC, ICC/IF
Diluizioni consigliate
WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reattività
Human, Mouse, Rat
Immunogeno
Recombinant fusion protein containing a sequence corresponding to amino acids 75-145 of human CHCHD2 (NP_057223.1).
Sequenza
TLGHAITGGFSGGSNAEPARPDITYQEPQGTQPAQQQQPCLYEIKQFLECAQNQGDIKLCEGFNEVLKQCR
Specie ospite
Rabbit
Clonalità
Polyclonal
Isotipo
IgG
Coniugare

Unconjugated

Purificazione
Affinity purification.
Peso molecolare
16 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
16.7kD protein, Aging Associated Gene 10 Protein, Aging-associated gene 10 protein, C7orf17, CHCH2_HUMAN, CHCHD 2, Coiled Coil Helix Coiled Coil Helix Domain Containing 2, Coiled coil helix coiled coil helix domain containing protein 2, mitochondrial, Coiled-coil-helix-coiled-coil-helix domain-containing protein 2, mitochondrial, HCV NS2 trans regulated protein, HCV NS2 trans-regulated protein, NS2TP
Controlli isotipo
Secondari adatti
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Western Blot - Anti-CHCHD2 Antibody (A88474) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 1: Western Blot - Anti-CHCHD2 Antibody (A88474)
Western blot analysis of extracts of various cell lines, using Anti-CHCHD2 Antibody (A88474) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Western Blot - Anti-CHCHD2 Antibody (A88474) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 2: Western Blot - Anti-CHCHD2 Antibody (A88474)
Western blot analysis of extracts from normal (control) and CHCHD2 knockout (KO) 293T cells, using Anti-CHCHD2 Antibody (A88474) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry - Anti-CHCHD2 Antibody (A88474) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 3: Immunohistochemistry - Anti-CHCHD2 Antibody (A88474)
Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-CHCHD2 Antibody (A88474) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-CHCHD2 Antibody (A88474) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 4: Immunohistochemistry - Anti-CHCHD2 Antibody (A88474)
Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-CHCHD2 Antibody (A88474) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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