Figure 1: Western Blot - Anti-Cdk4 Antibody (A305840)
Western blot analysis of extracts from normal (control) and CDK4 knockout (KO) HeLa cells, using Anti-Cdk4 Antibody (A305840) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Figure 2: Western Blot - Anti-Cdk4 Antibody (A305840)
Western blot analysis of extracts of various cell lines, using Anti-Cdk4 Antibody (A305840) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-Cdk4 Antibody (A305840) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse lung using Anti-Cdk4 Antibody (A305840) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-Cdk4 Antibody (A305840) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Figure 6: Western Blot - Anti-Cdk4 Antibody (A305840)
Immunoprecipitation analysis of 300µg extracts of HeLa cells using 3µg of Anti-Cdk4 Antibody (A305840). This Western blot was performed on the immunoprecipitate using Anti-Cdk4 Antibody (A305840) at a dilution of 1:1000.
![Western Blot - Anti-Cdk4 Antibody [ARC51004] (A81170) - Antibodies.com](https://cdn.antibodies.com/image/catalog/81/A81170_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-Cdk4 Antibody [IHC077] (A324523) - Antibodies.com](https://cdn.antibodies.com/image/catalog/324/A324523_1.jpg?profile=product_alternative)