Anti-CDK1 (phospho Tyr15) Anticorpo (A16400)

Name: Anti-CDK1 (phospho Tyr15) Antibody
Brand: Antibodies.com
SKU: A16400
Price: 435.0 USD
Availability: InStock

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$435

Anticorpo policlonale di coniglio contro CDK1 (phospho Tyr15) per WB, IHC, ICC/IF e IP.

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanzie

Garanzia di qualità al 100%

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Anti-CDK1 Anticorpo [POH-1]

Anticorpo monoclonale [POH-1] di topo contro CDK1 per IP, WB, IHC-P e ICC.

Descrizione Prodotto

Nome

Anti-CDK1 (phospho Tyr15) Antibody

Descrizione

Rabbit polyclonal antibody to CDK1 (phospho Tyr15).

Applicazioni

WB, IHC, ICC/IF, IP

Diluizioni consigliate

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:50-1:100

Reattività

Human, Mouse, Rat

Immunogeno

A synthetic phosphorylated peptide around Y15 of human CDK1 (NP_001777.1).

Sequenza

GTYGV

Specie ospite

Rabbit

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Affinity purification.

Peso molecolare

34 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

Cdc 2, Cdc2, CDC28A, CDK 1, CDK1_HUMAN, CDKN1, CELL CYCLE CONTROLLER CDC2, Cell division control protein 2, Cell division control protein 2 homolog, Cell division cycle 2 G1 to S and G2 to M, Cell division protein kinase 1, Cell Divsion Cycle 2 Protein, Cyclin Dependent Kinase 1, Cyclin-dependent kinase 1, DKFZp686L20222, MGC111195, p34 Cdk1, p34 protein kinase, P34CDC2

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (8)

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Figure 1: Western Blot - Anti-CDK1 (phospho Tyr15) Antibody (A16400)

Western blot analysis of extracts of NIH/3T3 cells, using Anti-CDK1 (phospho Tyr15) Antibody (A16400) at 1:1,000 dilution or CDK1 antibody (A0220). HeLa cellls were treated by nocodazole (50 ng/ml) at 37°C for 20 hours or Hydroxyurea (4 mM) at 37°C for 20 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-CDK1 (phospho Tyr15) Antibody (A16400)

Western blot analysis of extracts of various cell lines, using Anti-CDK1 (phospho Tyr15) Antibody (A16400) at 1:1,000 dilution. HeLa and NIH/3T3 cells were treated by nocodazole (50 ng/ml) at 37°C for 20 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 3: Western Blot - Anti-CDK1 (phospho Tyr15) Antibody (A16400)

Western blot analysis of extracts of C6 cells, using Anti-CDK1 (phospho Tyr15) Antibody (A16400) at 1:1,000 dilution. C6 cells were treated by Nocodazole (50 ng/ml) at 37°C for 20 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 4: Immunohistochemistry - Anti-CDK1 (phospho Tyr15) Antibody (A16400)

Immunohistochemistry analysis of paraffin-embedded rat liver using Anti-CDK1 (phospho Tyr15) Antibody (A16400) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.