This antibody recognizes an extracellular epitope of CD318, a type I transmembrane protein involved in early hematopoiesis.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: 1-4 µg/ml.
Reactivity
Human
Immunogen
NIH-3T3/CD318 cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
CUB1
Isotype
IgG2b
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
This antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Concentration
100 µg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Synonyms
CD318, CUB domain-containing protein 1, Membrane glycoprotein gp140, SIMA135, Subtractive immunization M plus HEp3-associated 135 kDa protein, Transmembrane and associated with src kinases, TRASK
Separation of HT-29 cells stained using Anti-CD318 Antibody (PE) (concentration in sample 5 µg/ml, red-filled) from HT-29 cells stained using Mouse IgG2b [MPC-11] (PE) isotype control (concentration in sample 5 µg/ml, same as CD318 PE concentration, black-dashed) in flow cytometry analysis (surface staining) of HT-29 cell suspension.
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