Mouse monoclonal [5E10] antibody to CD90/Thy1 (APC-Cyanine 7).
Specificità
This antibody recognizes CD90, a GPI-anchored cell surface glycoprotein expressed predominantly on thymocytes, hematopoietic stem cells, and neurons.
Applicazioni
Flow Cytometry
Diluizioni consigliate
Flow Cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reattività
Porcine, Horse, Human, Non-Human Primates
Reattività incrociata
This antibody does not cross react with Dog.
Immunogeno
HEL erythroleukemia cells.
Specie ospite
Mouse
Clonalità
Monoclonal
Clona ID
5E10
Isotipo
IgG1
Catene leggere
kappa
Coniugare
APC-Cyanine 7
Excitation: 650nm, Emission: 774nm
Purificazione
This antibody is conjugated with tandem dye APC-Cyanine 7 under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Conservazione
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Flow cytometric analysis of Jurkat cell suspension stained with Anti-CD90 Antibody [5E10] (APC-Cyanine 7) (4µl reagent per milion cells in 100µl of cell suspension).
Separation of Jurkat cells (red-filled) from SP2 cells (black-dashed) in flow cytometry analysis using Anti-CD90 Antibody [5E10] (APC-Cyanine 7) (4µl reagent per milion cells in 100µl of cell suspension).