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Anti-CD86 Anticorpo (A91282)

Nome
Anti-CD86 Antibody
Descrizione
Rabbit polyclonal antibody to CD86.
Applicazioni
WB, ICC/IF, IP
Diluizioni consigliate
WB: 1:100-1:500, ICC/IF: 1:50-1:200, IP: 1:50-1:200
Reattività
Human, Mouse, Rat
Immunogeno
Recombinant fusion protein containing a sequence corresponding to amino acids 24-247 of human CD86 (NP_787058.4).
Sequenza
APLKIQAYFNETADLPCQFANSQNQSLSELVVFWQDQENLVLNEVYLGKEKFDSVHSKYMGRTSFDSDSWTLRLHNLQIKDKGLYQCIIHHKKPTGMIRIHQMNSELSVLANFSQPEIVPISNITENVYINLTCSSIHGYPEPKKMSVLLRTKNSTIEYDGIMQKSQDNVTELYDVSISLSVSFPDVTSNMTIFCILETDKTRLLSSPFSIELEDPQPPPDHIP
Specie ospite
Rabbit
Clonalità
Polyclonal
Isotipo
IgG
Coniugare

Unconjugated

Purificazione
Affinity purification.
Peso molecolare
65 - 70 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
Activation B7-2 antigen, Activation B7-2 antigen 3, B-lymphocyte activation antigen B7-2, B-lymphocyte activation antigen B7-2 2, B7, B7 2, B7-2, B7.2, B70, B72 antigen, BU63, CD28 antigen ligand 2, CD28 antigen ligand 2 2, Cd28l2, CD28LG2, CD86 antigen, CD86 antigen (CD28 antigen ligand 2, B7-2 antigen), CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) 1, 2, CD86 molecule, CD86_HUMAN, CLS1, CTLA-4 counter-receptor B7.2, CTLA-4 counter-receptor B7.2 2, 3, Early T-cell costimulatory molecule 1, ETC-1, FUN 1, FUN-1, LAB72, Ly-58, Ly58, MB7, MB7-2, Membrane glycoprotein, MGC34413, T-lymphocyte activation antigen CD86, TS/A-2
Controlli isotipo
Secondari adatti
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Western Blot - Anti-CD86 Antibody (A91282) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 1: Western Blot - Anti-CD86 Antibody (A91282)
Western blot analysis of extracts of various cell lines, using Anti-CD86 Antibody (A91282) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.
Immunofluorescence - Anti-CD86 Antibody (A91282) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 2: Immunofluorescence - Anti-CD86 Antibody (A91282)
Immunofluorescence analysis of THP-1 cells using Anti-CD86 Antibody (A91282) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-CD86 Antibody (A91282) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 3: Immunofluorescence - Anti-CD86 Antibody (A91282)
Immunofluorescence analysis of rat spleen using Anti-CD86 Antibody (A91282) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-CD86 Antibody (A91282) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 4: Immunofluorescence - Anti-CD86 Antibody (A91282)
Immunofluorescence analysis of mouse spleen using Anti-CD86 Antibody (A91282) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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