Anti-CD80 Anticorpo [MEM-233] (FITC) (A281124)

Anti-CD80 Antibody [MEM-233] (FITC)

Mouse monoclonal [MEM-233] antibody to CD80 (FITC).

This antibody recognises human CD80, also known as B7-1, a ~60 kDa type 1 trans-membrane protein expressed of macrophages, dendritic cells and activated B-cells. CD80 is a member of the immunoglobulin superfamily having an extracellular domain bearing both a single Ig-v-like domain, a single Ig-c-like domain, a transmembrane sequence and a short cytoplasmic domain. Although the predicted molecular weight for human CD80 is ~33 kDa, the presence of multiple (8) potential N-glycosylation sites results in a migration corresponding to ~60 kDa.Human CD80 along with CD86 act as co-stimulatory molecules and are both ligands for CD28 and CTLA-4 involved in T cell activation and proliferation. Although CD80 binds to the same receptors as CD86 it displays quite different characteristics in its avidity and binding kinetics. Mutagenesis indicates residues in both the Ig-v-like and Ig-c-like domains of CD80 are crucial for the interaction with it’s receptors CTLA-4 and CD28. Mouse anti human CD80 antibody, clone MEM-233 binds to residues within the Ig-v-like domain of human CD80 as shown by domain switching assays. Mouse anti Human CD80, clone MEM-233 in combination with Mouse anti Human CD86, clone Bu63 (MCA1118) suggest that clone MEM-233 is able to block binding of human CD80 with it's cognate ligands CD28 and CTLA-4.

Flow Cytometry

Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 10⁶ cells or 100µl whole blood

Human

Mouse

Monoclonal

MEM-233

IgG1

FITC

Excitation: 490nm, Emission: 525nm

100 µg/ml

Liquid

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Mouse anti Human CD80 antibody, clone MEM-233 recognizes human CD80, also known as B7-1, a ~60 kDa type 1 trans-membrane protein expressed of macrophages, dendritic cells (Munro et al. 1994) and activated B-cells (Ranheim et al. 1993). CD80 is a member of the immunoglobulin superfamily having an extracellular domain bearing both a single Ig-v-like domain, a single Ig-c-like domain, a transmembrane sequence and a short cytoplasmic domain. Although the predicted molecular weight for human CD80 is ~33 kDa, the presence of multiple (8) potential N-glycosylation sites (Chen et al. 1998) results in a migration corresponding to ~60 kDa.Human CD80 along with CD86 act as co-stimulatory molecules and are both ligands for CD28 and CTLA-4 (Azuma et al. 1993) involved in T cell activation and proliferation (Vasu et al. 2003). Although CD80 binds to the same receptors as CD86 it displays quite different characteristics in its avidity and binding kinetics (van der Merwe et al. 1997).Mutagenesis indicates residues in both the Ig-v-like and Ig-c-like domains of CD80 are crucial for the interaction with it’s receptors CTLA-4 and CD28 (Peach et al. 1995). Mouse anti human CD80 antibody, clone MEM-233 binds to residues within the Ig-v-like domain of human CD80 as shown by domain switching assays (Vasu et al. 2003).Mouse anti Human CD80, clone MEM-233 in combination with Mouse anti Human CD86, clone Bu63 (MCA1118) suggest that clone MEM-233 is able to block binding of human CD80 with it's cognate ligands CD28 and CTLA-4 (Morbach et al. 2011).

Activation B7-1 antigen, B lymphocyte activation antigen B7, B7, B7-1, B7-1 antigen, B7.1, BB1, CD28 antigen ligand 1, CD28LG, CD28LG1, CD80 antigen, CD80 antigen (CD28 antigen ligand 1, B7-1 antigen), CD80 molecule, CD80_HUMAN, Costimulatory factor CD80, costimulatory molecule variant IgV-CD80, CTLA-4 counter-receptor B7.1, LAB7, T-lymphocyte activation antigen CD80

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