This antibody recognises the murine CD8 cell surface antigen, expressed by a subset of T lymphocytes.Rat anti Mouse CD8 antibody, clone YTS169.4 exhibits depleting activity when used in vivo.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 106 cells in 100µl. The Fc region of monoclonal antibodies may bind to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/BUF041B).
Reactivity
Mouse
Host
Rat
Clonality
Monoclonal
Clone ID
YTS169.4
Isotype
IgG2b
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with 1 ml of distilled water.
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.
Storage
Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Rat anti Mouse CD8 antibody, clone YTS169.4 recognizes the murine CD8 cell surface antigen, expressed by a subset of T lymphocytes.Rat anti Mouse CD8 antibody, clone YTS169.4 exhibits depleting activity when used in vivo.