This antibody recognizes a conformationally-dependent extracellular epitope of CD8, a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. CD8 is a disulfide-linked dimer and exists as a CD8 alpha/alpha homodimer or CD8 alpha/beta heterodimer (each monomer approx. 32-34 kDa). The antibody does not react with formaldehyde-fixed cells; negative in western blotting.
Applications
Flow Cytometry, IP, CyTOF
Dilutions
Flow Cytometry: 1 µg/ml.
Reactivity
Human
Immunogen
Crude thymus membrane fraction.
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-31
Isotype
IgG2a
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Predicted MW
32-34 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.