This antibody recognises the alpha-chain of human CD8, a 32kD glycoprotein expressed by the cytotoxic-suppressor subset of T cells and weakly by NK cells. CD8 functions as a receptor for MHC Class I molecules.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to label 1x106 cells in 100µl.
Reactivity
Human
Host
Mouse
Clonality
Monoclonal
Clone ID
Bu88
Isotype
IgG1
Conjugate
FITC
Excitation: 490nm, Emission: 525nm
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
100 µg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
General Notes
Mouse anti Human CD8 antibody, clone Bu88 recognizes the alpha-chain of human CD8, a 32kD glycoprotein expressed by the cytotoxic-suppressor subset of T cells and weakly by NK cells. CD8 functions as a receptor for MHC Class I molecules.