This antibody recognizes a protein of 33-55kDa, identified as CD53 (Workshop VI; Code N-L033). It is expressed on monocytes and macrophages, dendritic cells, osteoblasts and osteoclasts, and on T and B cells from every stage of differentiation but is absent from platelets, red blood cells. CD53 appears to be the marker with widest reactivity as well as the marker with the strictest specificity to hematopoietic cells. CD53 is a type III membrane with both termini in the cytoplasm and two loops in the extracellular environment. This molecule, in common with other members of tetraspan family, is involved in cellular activation as part of a signal transduction complex involving other membrane glycoproteins. CD53 crosslinking induces calcium flux on human monocyte and B cells. Cross-linking of CD53 promotes activation of resting human B-lymphocytes. This antibody recognizes CD53 transfected cells and partially inhibits T-cell proliferation induced by CD3 antibody (clone: UCHT1).

Applicazioni

Functional Studies, Flow Cytometry, IF

Diluizioni consigliate

Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml

Reattività

Human

Reattività incrociata

This antibody does not cross react with Baboon or Horse.

Immunogeno

Stimulated human leukocytes.

Specie ospite

Mouse

Clonalità

Monoclonal

Clona ID

161-2

Isotipo

IgG2a

Catene leggere

kappa

Coniugare

Unconjugated

Purificazione

Protein A/G chromatography.

Concentrazione

200 µg/ml

Peso molecolare

33-55 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Note generali

This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-CD53 Antibody [161-2] - BSA and Azide free (A253918).

Sinonimi

AI323659, Antigen MOX44 identified by monoclonal, CD 53, CD53 antigen, CD53 antigen, isoform CRA_b, CD53 glycoprotein, Cd53 molecule, CD53 tetraspan antigen, CD53_HUMAN, Cell surface glycoprotein CD53, Leukocyte antigen MRC OX-44, Leukocyte surface antigen CD53, MOX 44, MOX44, OTTHUMP00000059505, Ox-44, OX44, RATOX44, Tetraspanin 25, Tetraspanin-25, Tetraspanin25, Transmembrane glycoprotein, TSPAN 25, Tspan-25, TSPAN25

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (1)

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Figure 1: SDS-PAGE - Anti-CD53 Antibody [161-2] (A250738)

SDS-PAGE analysis of Anti-CD53 Antibody [161-2] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.