Anti-CD5 Anticorpo [YKIX322.3] (A282049)

Anti-CD5 Antibody [YKIX322.3]

Rat monoclonal [YKIX322.3] antibody to CD5.

This antibody recognises canine CD5, a 67 kDa cell surface type 1 transmembrane glycoprotein also known as lymphocyte antigen T1, Ly-1 or Leu-1. CD5 is expressed on the surface of T-cells and thymocytes, CD5 is also expressed by NK cells at low levels. Rat anti dog CD5, cloneYKIX322.3 was clustered as canine CD5 in the First Canine Leucocyte Antigen Workshop. In a study of 73 cases of canine chronic lymphocytic leukemia (CLL) CD5 expression was absent on all cases of B-cell CLL as defined by CD21 expression and lack of CD3 or other T cell antigen expression. Rat anti dog CD5 serves as a useful marker for the discrimination of canine leukemias of differing origins.

IP, Flow Cytometry, IHC-Fr

Flow Cytometry: 1:50 - 1:100, Use 10µl of the suggested working dilution to label 10⁶ cell in 100µl.

Canine

Concanavilin A activated canine peripheral blood cells.

Rat

Monoclonal

YKIX322.3

IgG2a

Unconjugated

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Rat anti Dog CD5 antibody, clone YKIX322.3 recognizes canine CD5, a 67 kDa cell surface type 1 transmembrane glycoprotein also known as lymphocyte antigen T1, Ly-1 or Leu-1. CD5 is expressed on the surface of T-cells and thymocytes, CD5 is also expressed by NK cells at low levels (Huang et al. 2008). Rat anti dog CD5, cloneYKIX322.3 was clustered as canine CD5 in the First Canine Leucocyte Antigen Workshop (Cobbold et al. 1994).In a study of 73 cases of canine chronic lymphocytic leukemia (CLL) CD5 expression was absent on all cases of B-cell CLL as defined by CD21 expression and lack of CD3 or other T cell antigen expression (Vernau and Moore 1999). Rat anti dog CD5 serves as a useful marker for the discrimination of canine leukemias of differing origins (Deravi et al. 2017).

CD 5, CD5 antigen, CD5 antigen (p56 62), CD5 molecule, CD5_HUMAN, LEU1, LyA, Lymphocyte antigen T1/Leu-1, OTTHUMP00000236973, T-cell surface glycoprotein CD5, T1