Mouse monoclonal [L17F12] antibody to CD5 (PE-DyLight® 594).
Specificità
This antibody reacts with an extracellular epitope of CD5, a 67kDa single-chain transmembrane glycoprotein expressed on mature T lymphocytes, most thymocytes, and B lymphocytes subset (B-1a lymphocytes).
Applicazioni
Flow Cytometry
Diluizioni consigliate
Flow Cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reattività
Human
Immunogeno
Human acute lymphoblastic leukemia (ALL) T cells.
Specie ospite
Mouse
Clonalità
Monoclonal
Clona ID
L17F12
Isotipo
IgG2a
Catene leggere
kappa
Coniugare
PE-DyLight® 594
Purificazione
This antibody is conjugated with tandem dye PE-DyLight® 594 under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
MW previsto
67 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Conservazione
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Flow cytometric analysis of human peripheral whole blood stained using Anti-CD5 Antibody [L17F12] (PE-DyLight 594) (4µl reagent per 100µl of peripheral whole blood).
Separation of human CD5 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis of human peripheral whole blood using Anti-CD5 Antibody [L17F12] (PE-DyLight 594) (4µl reagent per 100µl of peripheral whole blood).
![Flow Cytometry - Anti-CD5 Antibody [L17F12] (PE-DyLight® 594) (A121971) - Antibodies.com](https://cdn.antibodies.com/image/catalog/121/A121972_1.jpg?profile=product_top)
![Flow Cytometry - Anti-CD5 Antibody [L17F12] (PE-DyLight® 594) (A121972) - Antibodies.com](https://cdn.antibodies.com/image/catalog/121/A121972_2.jpg?profile=product_top)
![Flow Cytometry - Anti-CD5 Antibody [L17F12] (PE-DyLight® 594) (A121971) - Antibodies.com](https://cdn.antibodies.com/image/catalog/121/A121972_1.jpg?profile=product_top_thumb)
![Flow Cytometry - Anti-CD5 Antibody [L17F12] (PE-DyLight® 594) (A121972) - Antibodies.com](https://cdn.antibodies.com/image/catalog/121/A121972_2.jpg?profile=product_top_thumb)
![Flow Cytometry - Anti-CD5 Antibody [L17F12] (PE-DyLight® 594) (A121971) - Antibodies.com](https://cdn.antibodies.com/image/catalog/121/A121972_1.jpg?profile=product_image)
![Flow Cytometry - Anti-CD5 Antibody [L17F12] (PE-DyLight® 594) (A121972) - Antibodies.com](https://cdn.antibodies.com/image/catalog/121/A121972_2.jpg?profile=product_image)