This antibody recognises pig CD5. Clone 1H6/8 was clustered as CD5a at the Second International Swine CD antigen Workshop , based on its cellular distribution, ability to completely inhibit the binding of monoclonal antibody clone b53b7, the internal control for the second workshop and the immunoprecipitation of the same 55 kDa + 60 kDa doublet as the control antibody. Porcine CD5 is expressed by all circulating peripheral blood T lymphocytes and some B cells. CD5 is not expressed by porcine ?/d T cells and can hence be used for the discrimination between NK cells (CD4- CD8+ CD5-) and MHC-restricted cytotoxic T cells (CD4- CD8+ CD5+). No reactivity of Mouse anti Pig CD5 antibody, clone 1H6/8 was noted with canine, bovine, equine or human tissue samples (Doménech et al. 2003).
Applicazioni
WB, IP, Flow Cytometry
Diluizioni consigliate
Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to 1x106 cells in 100µl
Reattività
Porcine
Immunogeno
Con A / PMA activated porcine peripheral blood cells.
Specie ospite
Mouse
Clonalità
Monoclonal
Clona ID
1H6/8
Isotipo
IgG2a
Coniugare
FITC
Excitation: 490nm, Emission: 525nm
Purificazione
Protein G affinity chromatography of tissue culture supernatant.
Concentrazione
100 µg/ml
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.
Conservazione
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Note generali
Mouse anti Pig CD5 antibody, clone 1H6/8 recognizes pig CD5. Clone 1H6/8 was clustered as CD5a at the Second International Swine CD antigen Workshop (Saalmuller et al. 1998), based on its cellular distribution, ability to completely inhibit the binding of monoclonal antibody clone b53b7, the internal control for the second workshop and the immunoprecipitation of the same 55 kDa + 60 kDa doublet as the control antibody (Pescowitz et al. 1998). Porcine CD5 is expressed by all circulating peripheral blood T lymphocytes and some B cells. CD5 is not expressed by porcine ?/d T cells (Saalmuller et al. 1994) and can hence be used for the discrimination between NK cells (CD4- CD8+ CD5-) and MHC-restricted cytotoxic T cells (CD4- CD8+ CD5+). No reactivity of Mouse anti Pig CD5 antibody, clone 1H6/8 was noted with canine, bovine, equine or human tissue samples (Doménech et al. 2003).
