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Anti-CD47 Anticorpo (A13692)

Nome
Anti-CD47 Antibody
Descrizione
Rabbit polyclonal antibody to CD47.
Applicazioni
WB, IHC, ICC/IF
Diluizioni consigliate
WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reattività
Human, Mouse, Rat
Immunogeno
A synthetic peptide corresponding to a sequence within amino acids 224-323 of human CD47 (NP_001768.1).
Sequenza
HYYVFSTAIGLTSFVIAILVIQVIAYILAVVGLSLCIAACIPMHGPLLISGLSILALAQLLGLVYMKFVASNQKTIQPPRKAVEEPLNAFKESKGMMNDE
Specie ospite
Rabbit
Clonalità
Polyclonal
Isotipo
IgG
Coniugare

Unconjugated

Purificazione
Affinity purification.
Peso molecolare
47 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
Antigen identified by monoclonal, Antigenic surface determinant protein OA3, CD 47, CD47 antigen, CD47 antigen (Rh-related antigen, integrin-associated signal transducer), CD47 glycoprotein, CD47 molecule, CD47_HUMAN, IAP, Integrin Associated Protein, Integrin associated signal transducer, Integrin-associated protein, Leukocyte surface antigen CD47, MER 6, MER6, OA 3, OA3, OTTHUMP00000041152, OTTHUMP00000041153, Protein MER6, Rh related antigen, Surface antigen identified by monoclonal
Controlli isotipo
Secondari adatti
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Western Blot - Anti-CD47 Antibody (A13692) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 1: Western Blot - Anti-CD47 Antibody (A13692)
Western blot analysis of extracts of various cell lines, using Anti-CD47 Antibody (A13692) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Western Blot - Anti-CD47 Antibody (A13692) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 2: Western Blot - Anti-CD47 Antibody (A13692)
Western blot analysis of extracts of U-251MG cells, using Anti-CD47 Antibody (A13692) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry - Anti-CD47 Antibody (A13692) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 3: Immunohistochemistry - Anti-CD47 Antibody (A13692)
Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-CD47 Antibody (A13692) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-CD47 Antibody (A13692) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 4: Immunohistochemistry - Anti-CD47 Antibody (A13692)
Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-CD47 Antibody (A13692) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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