This antibody recognises the murine CD40 ligand, also known as CD40L or CD154. CD154 is a ~39 kDa cell surface glycoprotein. CD154 is expressed by activated CD4+ve T lymphocytes. Hamster anti Mouse CD154 antibody, clone 39H5 is routinely tested in flow cytometry on mouse CD40L transfected L cells.

Applicazioni

Flow Cytometry

Diluizioni consigliate

Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl. The Fc region of monoclonal antibodies may bind to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/BUF041B).

Reattività

Mouse

Immunogeno

L cells transfected with CD40L.

Specie ospite

Hamster

Clonalità

Monoclonal

Clona ID

39H5

Isotipo

IgG

Coniugare

FITC

Excitation: 490nm, Emission: 525nm

Purificazione

Protein G affinity chromatography of tissue culture supernatant.

Concentrazione

100 µg/ml

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Conservazione

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Note generali

Hamster anti Mouse CD154 antibody, clone 39H5 recognizes the murine CD40 ligand, also known as CD40L or CD154. CD154 is a ~39 kDa cell surface glycoprotein . CD154 is expressed by activated CD4+ve T lymphocytes. Hamster anti Mouse CD154 antibody, clone 39H5 is routinely tested in flow cytometry on mouse CD40L transfected L cells.

Sinonimi

CD 40L, CD154, CD40 antigen ligand, CD40 ligand, CD40 ligand, soluble form, CD40-L, CD40LG, CD40L_HUMAN, gp39, hCD40L, HIGM1, IGM, IMD3, soluble form, T B cell activating molecule, T BAM, T cell antigen Gp39 , T-cell antigen Gp39, TNF related activation protein, TNF-related activation protein, TNFSF5, TrAP, Tumor necrosis factor (ligand) superfamily member 5, Tumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome), Tumor necrosis factor ligand superfamily member 5

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.