Anti-CD3G Anticorpo [ARC2105] (A306075)

Name: Anti-CD3G Antibody [ARC2105]
Brand: Antibodies.com
SKU: A306075
Price: 470.0 USD
Availability: InStock

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$470

Anticorpo monoclonale [ARC2105] di coniglio contro CD3G per WB, IHC, ICC/IF e Flow Cytometry.

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Descrizione Prodotto

Nome

Anti-CD3G Antibody [ARC2105]

Descrizione

Rabbit monoclonal [ARC2105] antibody to CD3G.

Applicazioni

WB, IHC, ICC/IF, Flow Cytometry

Diluizioni consigliate

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, Flow Cytometry: 1:50-1:200

Reattività

Human, Rat

Immunogeno

A synthetic peptide corresponding to a sequence within amino acids 83-182 of human CD3G (P09693).

Sequenza

GMYQCKGSQNKSKPLQVYYRMCQNCIELNAATISGFLFAEIVSIFVLAVGVYFIAGQDGVRQSRASDKQTLLPNDQLYQPLKDREDDQYSHLQGNQLRRN

Specie ospite

Rabbit

Clonalità

Monoclonal

Clona ID

ARC2105

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Affinity purification.

Peso molecolare

20 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

CD3 gamma, CD3-GAMMA, CD3g antigen gamma polypeptide, CD3g antigen gamma polypeptide (TiT3 complex), CD3g molecule gamma, CD3g molecule gamma (CD3 TCR complex), CD3G_HUMAN, FLJ17620, FLJ17664, FLJ79544, FLJ94613, MGC138597, T cell antigen receptor complex gamma subunit of T3, T-cell receptor T3 gamma chain, T-cell surface glycoprotein CD3 gamma chain, T3G

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (4)

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Figure 1: Western Blot - Anti-CD3G Antibody [ARC2105] (A306075)

Western blot analysis of extracts of various cell lines, using Anti-CD3G Antibody [ARC2105] (A306075) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 120s.

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Figure 2: Immunofluorescence - Anti-CD3G Antibody [ARC2105] (A306075)

Immunofluorescence analysis of Jurkat cells using Anti-CD3G Antibody [ARC2105] (A306075) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 3: Flow Cytometry - Anti-CD3G Antibody [ARC2105] (A306075)

Flow cytometry analysis of Jurkat cells, stained with Rabbit IgG isotype control (2.5 µg/ml, blue line) or Anti-CD3G Antibody [ARC2105] (A306075), (2.5 µg/ml orange line), followed by goat anti-Rabbit polyclonal antibody FITC (1:200 dilution) staining. Non-fluorescently stained Jurkat cells was used as blank control (red line).

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Figure 4: Flow Cytometry - Anti-CD3G Antibody [ARC2105] (A306075)

Flow cytometry analysis of Raji cells, stained with Rabbit IgG isotype control (2.5 µg/ml, blue line) or Anti-CD3G Antibody [ARC2105] (A306075), (2.5 µg/ml orange line), followed by goat anti-Rabbit polyclonal antibody FITC (1:200 dilution) staining. Non-fluorescently stained Raji cells was used as blank control (red line).