This antibody recognises the murine CD34 cell surface antigen, expressed by endothelial cells and by hematopoietic stem cells. recognises a neuraminidase sensitive epitope. As in the human system, CD34 antibodies in the mouse demonstrate slightly different staining patterns depending on their fine specificity. Rat anti Mouse CD34 antibody, clone MEC14.7 appears to recognize a subset of the stem cell population recognized by clone RAM34, and it is thought that this is due to differences in the epitope recognized by the two antibodies.
Applicazioni
IHC-P, IP, Flow Cytometry
Diluizioni consigliate
Flow Cytometry: 1:10 for 0.25 mg, 25 µg, 1:25 for 0.1 mg - 1:20 for 0.25 mg, 25 µg, 1:50 for 0.1 mg, IHC-P: 1:20 - 1:200, IP: 2 µg/ml - 10 µg/ml, Use 10µl of the suggested working dilution to label 106 cells in 100µl
Reattività
Mouse
Immunogeno
T-end.1, a pMT transformed endothelial cell line.
Specie ospite
Rat
Clonalità
Monoclonal
Clona ID
MEC14.7
Isotipo
IgG2a
Coniugare
Unconjugated
Purificazione
Protein G affinity chromatography of tissue culture supernatant.
Concentrazione
1 mg/ml
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline.
Conservazione
Store undiluted at -20°C only. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.
Note generali
Rat anti Mouse CD34 antibody, clone MEC14.7 recognizes the murine CD34 cell surface antigen, expressed by endothelial cells and by hematopoietic stem cells. Rat anti Mouse CD34 antibody, clone MEC14.7 recognizes a neuraminidase sensitive epitope. As in the human system, CD34 antibodies in the mouse demonstrate slightly different staining patterns depending on their fine specificity. Rat anti Mouse CD34 antibody, clone MEC14.7 appears to recognize a subset of the stem cell population recognized by clone RAM34, and it is thought that this is due to differences in the epitope recognized by the two antibodies.
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