Recombinant rabbit monoclonal [DM135] antibody to CD34.
Applicazioni
ELISA, Flow Cytometry
Diluizioni consigliate
ELISA: 1:5,000-10,000, Flow Cytometry: 1:100
Reattività
Human
Specie ospite
Rabbit
Clonalità
Monoclonal
Clona ID
DM135
Isotipo
IgG
Coniugare
Unconjugated
Purificazione
Affinity chromatography.
Concentrazione
Reconstitution dependent.
Forma del prodotto
Lyophilized
Ricostituzione
Reconstitute with distilled sterile water.
Formulazione
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Conservazione
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Note generali
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
ELISA plate pre-coated by 1 µg/ml (100 µl/well) Recombinant Human CD34 Protein (6×His Tag) (A318258) can bind Anti-CD34 Antibody [DM135] - Azide free (A318572) in a linear range of 0. 1-12 µg/ml.
Flow cytometry analysis with Anti-CD34 Antibody [DM135] - Azide free (A318572) on Expi293 cells transfected with human CD34 (blue histogram) or Expi293 transfected with irrelevant protein (red histogram).
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