Mouse monoclonal [4H11[APG]] antibody to CD34 (PE).
Specificità
This antibody reacts with extracellular class III epitope on CD34, a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells. It is gradually lost on progenitor cells. This antibody completely blocks binding of class II antibody QBEnd10 and class III antibodies BIRMA K3 and 8G12 on KG1a cell line.
Applicazioni
Flow Cytometry, ICC
Diluizioni consigliate
Flow Cytometry: The reagent is designed for analysis of human blood cells using 20 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Reattività
Human
Immunogeno
Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia.
Specie ospite
Mouse
Clonalità
Monoclonal
Clona ID
4H11[APG
Isotipo
IgG1
Coniugare
PE
Excitation: 565nm, Emission: 578nm
Purificazione
This antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
MW previsto
110-115 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Conservazione
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!