This antibody reacts with extracellular class III epitope on CD34, a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells. It is gradually lost on progenitor cells. This antibody completely blocks binding of class II antibody QBEnd10 and class III antibodies BIRMA K3 and 8G12 on KG1a cell line.

Applicazioni

Flow Cytometry

Diluizioni consigliate

Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reattività

Human

Immunogeno

Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia.

Specie ospite

Mouse

Clonalità

Monoclonal

Clona ID

4H11[APG

Isotipo

IgG1

Coniugare

APC

Excitation: 645nm, Emission: 660nm

Purificazione

This antibody is conjugated with Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.

MW previsto

110-115 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.

Conservazione

Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!

Sinonimi

CD34 antigen, CD34 molecule, CD34_HUMAN, Cluster designation 34, Hematopoietic progenitor cell antigen CD34, HPCA1, Mucosialin, OTTHUMP00000034733, OTTHUMP00000034734

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