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Anti-CD33 Anticorpo (A13841)

Nome
Anti-CD33 Antibody
Descrizione
Rabbit polyclonal antibody to CD33.
Applicazioni
WB, IHC, ICC/IF
Diluizioni consigliate
WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reattività
Human, Mouse, Rat
Immunogeno
Recombinant fusion protein containing a sequence corresponding to amino acids 49-259 of human SIGLEC3/CD33 (NP_001763.3).
Sequenza
YYDKNSPVHGYWFREGAIISRDSPVATNKLDQEVQEETQGRFRLLGDPSRNNCSLSIVDARRRDNGSYFFRMERGSTKYSYKSPQLSVHVTDLTHRPKILIPGTLEPGHSKNLTCSVSWACEQGTPPIFSWLSAAPTSLGPRTTHSSVLIITPRPQDHGTNLTCQVKFAGAGVTTERTIQLNVTYVPQNPTTGIFPGDGSGKQETRAGVVH
Specie ospite
Rabbit
Clonalità
Polyclonal
Isotipo
IgG
Coniugare

Unconjugated

Purificazione
Affinity purification.
Peso molecolare
70 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
CD 33, CD33 antigen, CD33 antigen (gp67), CD33 molecule, CD33_HUMAN, FLJ00391, gp67, My9, Myeloid cell surface antigen CD33, Myeloid cell surface antigen CD33 precursor, Myeloid differentiation antigen CD33, p67, Sialic acid binding Ig like lectin 3, Sialic acid binding immunoglobulin like lectin 3, Sialic acid-binding Ig-like lectin 3, SIGLEC 3, Siglec-3, SIGLEC3
Controlli isotipo
Secondari adatti
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Western Blot - Anti-CD33 Antibody (A13841) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 1: Western Blot - Anti-CD33 Antibody (A13841)
Western blot analysis of extracts of various cell lines, using Anti-CD33 Antibody (A13841) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.
Immunohistochemistry - Anti-CD33 Antibody (A13841) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 2: Immunohistochemistry - Anti-CD33 Antibody (A13841)
Immunohistochemistry analysis of paraffin-embedded mouse spleen using Anti-CD33 Antibody (A13841) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence - Anti-CD33 Antibody (A13841) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 3: Immunofluorescence - Anti-CD33 Antibody (A13841)
Immunofluorescence analysis of K-562 cells using Anti-CD33 Antibody (A13841) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-CD33 Antibody (A13841) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 4: Immunofluorescence - Anti-CD33 Antibody (A13841)
Immunofluorescence analysis of RAW264. 7 cells using Anti-CD33 Antibody (A13841) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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