Anti-CD31 Anticorpo [ER-MP12] (PE) (A280365)

Anti-CD31 Antibody [ER-MP12] (PE)

Rat monoclonal [ER-MP12] antibody to CD31 (PE).

This antibody recognises mouse CD31, a 140 kDa cell surface glycoprotein expressed at high levels on endothelial cells, platelets and most leukocyte subpopulations.CD31 is also expressed on a major population of macrophage / dendritic cell precursors in the bone marrow. Rat anti Mouse CD31 antibody, clone ER-MP12 can be used in conjunction with clone ER-MP20 (MCA2389GA) in two colour flow cytometric analysis, to identify different stages of myeloid progenitor cells in mouse bone marrow.

Flow Cytometry

Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl. The Fc region of monoclonal antibodies may bind to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/BUF041B).

Mouse

BALB/c macrophage precursor cell hybrids.

Rat

Monoclonal

ER-MP12

IgG2a

Protein G affinity chromatography of tissue culture supernatant.

Reconstitution dependent.

Lyophilized

Reconstitute with 1 ml of distilled water.

Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.

Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Rat anti Mouse CD31 antibody, clone ER-MP12 recognizes mouse CD31, a 140 kDa cell surface glycoprotein expressed at high levels on endothelial cells, platelets and most leukocyte subpopulations.CD31 is also expressed on a major population of macrophage / dendritic cell precursors in the bone marrow. Rat anti Mouse CD31 antibody, clone ER-MP12 can be used in conjunction with clone ER-MP20 (MCA2389GA) in two colour flow cytometric analysis, to identify different stages of myeloid progenitor cells in mouse bone marrow (de Bruijn et al. 1998).

Adhesion molecule, CD31 antigen, CD31 EndoCAM, EndoCAM, FLJ34100, FLJ58394, GPIIA, GPIIA', GPIIA'', HEC7, Leukocyte surface protein, PECA1, PECA1_HUMAN, PECAM, PECAM 1, PECAM 1 CD31 EndoCAM, PECAM-1, Pecam1, Platelet and endothelial cell adhesion molecule 1, Platelet endothelial cell adhesion molecule, Platelet/endothelial cell adhesion molecule, Platelet/endothelial cell adhesion molecule 1

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