Anti-CD3 Anticorpo [MEM-57] (A86068)

Anti-CD3 Antibody [MEM-57] - Low endotoxin, Azide free

Mouse monoclonal [MEM-57] antibody to CD3.

This antibody reacts with an extracellular epitope on gamma-epsilon and delta-epsilon dimers of human CD3 complex, a part of a bigger multi-subunit T cell receptor complex (CD3/TCR) expressed on peripheral blood T lymphocytes and mature thymocytes.

Flow Cytometry, IP, CyTOF, Functional Studies

IP: The antibody MEM-57 immunoprecipitates from a detergent lysate of surface-radioiodinated T cells a strong zone of about 22 kDa and a weak 28-kDa zone, which is typical pattern yielded by a reference antibody Leu-4 (SK7), FUNC: This antibody has strong mitogenic effect on peripheral T lymphocytes, it reacts strongly with gamma/delta T lymphocytes, Flow Cytometry: 1-4 µg/ml; Positive Control: peripheral blood lymphocytes, JURKAT human leukemia T cell line.

Human

Human thymocytes and T lymphocytes.

Mouse

Monoclonal

MEM-57

IgG2a

kappa

Unconjugated

Protein A chromatography.

Liquid

Supplied in Phosphate Buffered Saline, pH 7.4, without Sodium Azide (0.2 µm filter sterilized). Endotoxin level is less than 0.01 EU/µg of the protein.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

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